The liver is important for protein synthesis, detoxification, and amino acid metabolism. Nitric oxide (NO) is a key mediator in hepatocyte proliferation during liver regeneration. It is an oxidative metabolite of L-arginine produced by enzymes called nitric oxide synthase. The aim of this study is to evaluate the effect of L-arginine on the liver functions of patients with liver disorders and to explore the role of L-arginine in the pathogenesis of the liver. The biochemical tests of the liver like ALT, AST, and ALP were done in the groups. The treatment with L-arginine as a test drug showed a significant decrease in the levels of biochemical markers to the near-normal levels. The study concluded that the treatment with formulations containing L-arginine helped in regaining the normal hepatic architecture.