Non-small cell lung cancer (NSCLC) is the prevailing kind of lung cancer. Molecular target has exceptionally improved the treatment viability of lung cancer, however, new difficulties have developed, for example, drug resistance and cancer repeat. Along these lines, new chemotherapeutic operators and treatment techniques are earnestly required. Reserpine, a natural indole alkaloid, is the fundamental dynamic piece of Indian restorative plant which has been appeared to show ground-breaking hostile to cancer action in specific kinds of cancer; be that as it may, its movement in drug-safe lung cancer has never been tended to. In this investigation, we utilized 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) measure and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) test to examine reserpine anticancer movement. The cytotoxicity of reserpine in NSCLC cell lines was profoundly investigated. Reserpine displayed specific cytotoxicity among NSCLC at 15, 25 and 35 µM concentrations. Reserpine essentially expanded the action of ROS, which are profoundly expanded apoptosis. Improved ROS, altogether initiated customized cell passing in human NSCLC cells. The upgrade of ROS age in drug-safe NSCLC cells prompts impedance of development and acceptance of apoptosis. In this way, reserpine-prompted cell apoptosis is firmly connected with ROS rise in the cells. These discoveries show that reserpine can be a powerful mix of treating drug-safe NSCLC.